Recombinant AntibodiesMonoclonal Antibodies
- Sequencing of hybridomas – identification of heavy and light chain sequences –
- Gene synthesis of the heavy and light chains
- Transfection in mamalian expression vector (CHO cells)
- Studies of transient expression and generation of stable cell lines
- Purification and characterisation of recombinant MAbs
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Murine Hybridomas and Mabs
- Generation of murine hybridoma clones
- Animal immunization
- Fusion, selection, cloning and expansion of hybridomas
- Screening, characterization and isotyping of Mabs
- In vitro production of MAbs at different scales
- Bioreactors in static and continuous perfusion cultures
- Purification of Mabs
- Affinity purification